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Preliminary results on genome mapping of an Italia x Mercan grapevine population

This research was conducted to construct genetic linkage maps of Vitis (2n = 38) from grape populations (60 F1 + 2 parents) of crossing of Italia and Mercan (Vitis vinifera L.) cultivars. RAPD reactions were performed using a total of 300 RAPD primers. | Turk J Agric For 30 (2006) 273-280 © TÜB‹TAK Preliminary Results on Genome Mapping of an Italia x Mercan Grapevine Population Zeliha GÖKBAYRAK1, Cengiz ÖZER2, Gökhan SÖYLEMEZO⁄LU3,* 1 Çanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Horticulture, 17020 Çanakkale - TURKEY 2 Tekirda¤ Viticultural Research Institute, Tekirda¤ - TURKEY 3 Ankara University Faculty of Agriculture Department of Horticulture, 06110 Ankara - TURKEY Received: 09.02.2006 Abstract: This research was conducted to construct genetic linkage maps of Vitis (2n = 38) from grape populations (60 F1 + 2 parents) of crossing of Italia and Mercan (Vitis vinifera L.) cultivars. RAPD reactions were performed using a total of 300 RAPD primers. The amplification products were scored from negatives as presence or absence. Only 59 loci for Italia and 55 loci for Mercan could be used for linkage analysis. Mapmaker/Exp.3.0 was used for genetic linkage analysis with multipoint ordering among markers at LOD ≥ 3.0 score. The map was developed by using the double pseudo-testcross mapping approach. Italia and Mercan resulted in 8 (maternal) and 6 (paternal) linkage groups, respectively. Loci placed on the linkage groups were further analyzed by regression and analyses of variances in order to determine possible linkages between the loci and morphological and disease resistance characteristics. According to the results, only 2 characters, flowering time and resistance to powdery mildew, were significantly linked (P ≥ 0.01) to 1 and 2 marker loci, respectively. Other significant (P ≥ 0.05) marker-QTL relationships were also found for various traits from regression analysis. Key Words: Grape, genetic map, linkage analysis, QTL, RAPD Italia x Mercan Asma Populasyonunda Genom Haritalamas› Üzerine ‹lk Sonuçlar Özet: Bu araflt›rmada, V. vinifera L. varyetesi olan Italia ve Mercan üzüm çeflitlerinin melezlenmesi ile elde edilen F1 populasyonu kullan›larak (60 F1 + 2 ebeveyn) genom haritas›n›n .