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RAPD analysis of genetic variation in barley

The variation between wild barley lines (Hordeum murinum, Hordeum bulbosum and Hordeum vulgare spontaneum) originated from Turkey were investigated comparatively at the molecular level with the Random Amplified Polymorphic DNA (RAPD) technique. | Tr. J. of Agriculture and Forestry 23 (1999) 627-630 © TÜBİTAK RAPD Analysis of Genetic Variation in Barley Gülruh ALBAYRAK Istanbul University, Faculty of Science, Biology Department, 34459 Vezneciler, Istanbul-TURKEY Nermin GÖZÜKIRMIZI Istanbul University, Faculty of Science, Biology Department, 34459 Vezneciler, İstanbul-TURKEY Received: 08.06.1998 Abstract: The variation between wild barley lines (Hordeum murinum, Hordeum bulbosum and Hordeum vulgare spontaneum) originated from Turkey were investigated comparatively at the molecular level with the Random Amplified Polymorphic DNA (RAPD) technique. Leaf DNAs extracted from different barley lines were amplified with randomly chosen primers in a Polymerase Chain Reaction (PCR). Rates of polymorphisms between the 23 lines were determined in pairwise comparisons. The lowest value of polymorphism was determined to be 0.3% between wild barley lines numbered 50327 and 50354, and between 48101 and 50365 while the highest polymorphism value was determined to be 60% between wild barley lines numbered 40659 and 49047. The results can be used in the selection of commercially important traits (such as resistance against disease, drought etc.) present in wild barley lines. Thus, development of high quality barley cultivars will be possible. Arpada Genetik Varyasyonun RAPD Analizi Özet: Türkiye kökenli yabani arpa hatları (Hordeum murinum, Hordeum bulbosum ve Hordeum vulgare spontaneum) arasındaki çeşitlilik moleküler düzeyde Rastgele Çoğaltılmış Polimorfik DNA (RAPD) tekniği kullanılarak karşılaştırmalı olarak araştırılmıştır. Farklı arpa hatlarından ekstre edilen yaprak DNA’ları Polimeraz Zincir Reaksiyonu’nda (PCR) rastgele seçilmiş primerler ile çoğaltıldı. 23 hat arasındaki polimorfizm oranı çiftler halinde karşılaştırmalı olarak tayin edildi. En yüksek polimorfizm oranı %60 olarak 40659 ile 49047 nolu yabani arpa hatları arasında bulunurken en düşük polimorfizm oranı ise %0.3 olarak 50327 ile 50354, ve 48101 ile .