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Cytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell line

The main purpose of this study was to analyze the cytotoxic activity of an extract obtained from Centaurea fenzlii Reichardt, and the fractions eluted from this extract, in breast cancer cells. After isolation and structural analysis of the fractions were conducted, a meaningful cytotoxic effect was indicated. | Turkish Journal of Biology Turk J Biol (2017) 41: 370-377 © TÜBİTAK doi:10.3906/biy-1609-74 http://journals.tubitak.gov.tr/biology/ Research Article Cytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell line 1 2 3 4, Ümit YIRTICI , Fatih GÖGER , Mehmet SARIMAHMUT , Aysun ERGENE * Department of Medical Laboratory, School of Health Care, Kırıkkale University, Kırıkkale, Turkey 2 Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey 3 Department of Biology, Faculty of Science and Arts, Uludağ University, Bursa, Turkey 4 Department of Biology, Faculty of Science and Art, Kırıkkale University, Kırıkkale, Turkey 1 Received: 29.09.2016 Accepted/Published Online: 02.12.2016 Final Version: 20.04.2017 Abstract: The main purpose of this study was to analyze the cytotoxic activity of an extract obtained from Centaurea fenzlii Reichardt, and the fractions eluted from this extract, in breast cancer cells. After isolation and structural analysis of the fractions were conducted, a meaningful cytotoxic effect was indicated. The goal of the analysis was to reveal the mechanism by which this effect occurs through researching the apoptotic side of these fractions and determining the amount of several proteins that are the products of the genes. Test substances were applied to breast cancer cells and the inhibitory concentration value 50 (IC50) that caused a cytotoxic effect was determined using MTT and ATP assays. The Centaurea fenzlii Reichardt dichloromethane extracts-ethyl acetate fractions (CFDCMEAF) exhibited a stronger growth-inhibitory effect on MCF-7 cells (45.771 µg/mL). The apoptotic effect was studied using double staining and flow cytometry. The death rate in the cells treated with the CFDCM-EAF IC50 dose was approximately 90%: 9.2% living cells, 22.8% necrotic cells, 62.3% late apoptotic cells, and 5.8% early apoptotic cells. Structural analysis of the CFDCM-EAF, .