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Efficient polyhydroxybutyrate production from Bacillus thuringiensis using sugarcane juice substrate

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The present study focused on the screening and optimization of biopolymer polyhydroxybutyrate (PHB) production by Bacillus spp. using cost-effective substrates. | Turkish Journal of Biology http://journals.tubitak.gov.tr/biology/ Research Article Turk J Biol (2017) 41: 992-1002 © TÜBİTAK doi:10.3906/biy-1704-13 Efficient polyhydroxybutyrate production from Bacillus thuringiensis using sugarcane juice substrate 1,2, 1 1,2 Anon THAMMASITTIRONG *, Sudarat SAECHOW , Sutticha Na-Ranong THAMMASITTIRONG Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University, Nakhon Pathom, Thailand 2 Microbial Biotechnology Research Unit, Faculty of Liberal Arts and Science, Kasetsart University, Nakhon Pathom, Thailand 1 Received: 07.04.2017 Accepted/Published Online: 29.10.2017 Final Version: 18.12.2017 Abstract: The present study focused on the screening and optimization of biopolymer polyhydroxybutyrate (PHB) production by Bacillus spp. using cost-effective substrates. Among 602 local Bacillus isolates, Bacillus thuringiensis B417-5 produced the highest amount of PHB (2.278 g/L, 60.07% of dry cell weight, DCW). 1H NMR and FTIR analyses of the extracted polymer revealed the characteristic peaks of PHB. The optimization results showed that the highest PHB accumulation (2.768 g/L, 72.08% of DCW) was achieved when culturing B. thuringiensis B417-5 in a nitrogen-deficient medium containing 1% total sugar from sugarcane juice and 0.5% yeast extract, with a pH of 7.0 and an incubation temperature of 37 °C for 48 h. B. thuringiensis B417-5 can thus be considered a good candidate for large-scale production of PHB. We are reporting for the first time that sugarcane juice is a promising carbon source for economical PHB production by B. thuringiensis. Key words: Bacillus thuringiensis, bioplastic, polyhydroxyalkanoate, polyhydroxybutyrate, sugarcane juice 1. Introduction Extensive use of petroleum-derived plastics (approximately 269 million tons used globally in 2015) increases the environmental concerns of nonbiodegradable wastes, including contamination with small fragments of toxic compounds leaching out of .