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Molecular cytogenetic identification of a novel hexaploid wheat–Thinopyrum intermedium partial amphiploid with high protein content

Genomic in situ hybridization and fluorescence in situ hybridization were used to define the cytogenetic constitution of the intergeneric introgression line 29(1-57) obtained from the wheat × Thinopyrum intermedium (wheatgrass) cross. | Turkish Journal of Biology Turk J Biol (2016) 40: 554-560 © TÜBİTAK doi:10.3906/biy-1503-30 http://journals.tubitak.gov.tr/biology/ Research Article Molecular cytogenetic identification of a novel hexaploid wheat–Thinopyrum intermedium partial amphiploid with high protein content 1 1 2 1 2, Mariyana GEORGIEVA , Klaudia KRUPPA , Nedyalka TYANKOVA , Márta MOLNÁR-LÁNG * Department of Molecular Genetics, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria 2 Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary Received: 10.03.2015 Accepted/Published Online: 09.06.2015 Final Version: 18.05.2016 Abstract: Genomic in situ hybridization and fluorescence in situ hybridization were used to define the cytogenetic constitution of the intergeneric introgression line 29(1-57) obtained from the wheat × Thinopyrum intermedium (wheatgrass) cross. This line is a highprotein line resistant to leaf rust, yellow rust, and powdery mildew under field conditions and has 42 chromosomes. GISH analysis using a total genomic DNA probe from Thinopyrum intermedium resulted in the identification of 14 chromosomes of wheatgrass origin. The sequential GISH patterns obtained using St-genomic and J-genomic DNA as probes and ABD-genomic DNA from wheat as a blocker led to a better determination of the genomic type of wheatgrass chromosomes. Rearrangements involving Thinopyrum chromosomes and the A, B, and D genomes of wheat were not observed. FISH using the repetitive DNA probes pSc119.2, Afa-family, and pTa71 allowed for identification of all the wheat chromosomes present. Key words: FISH, GISH, wheat–Thinopyrum intermedium introgression line 1. Introduction The use of alien germplasm has increased intensively in cereal breeding programs for wheat improvement (Whitford et al., 2013). The alien species involved in the development of new amphiploids and wheat–alien chromosome addition, .