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Identification, characterization, and expression profiling of salt-stress tolerant proton gradient regulator 5 (PGR5) in Gossypium arboreum

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Salinity is among the significant abiotic stresses adversely affecting plant development and yield for a wide range of crops. For quantitative and qualitative expression studies of proton gradient regulator 5 (PGR5) under salt stress, differential display RT-PCR and rapid amplification of cDNA ends (RACE) were performed on salt-stress responsive Gossypium arboreum. | Turkish Journal of Biology Turk J Biol (2016) 40: 889-898 © TÜBİTAK doi:10.3906/biy-1504-30 http://journals.tubitak.gov.tr/biology/ Research Article Identification, characterization, and expression profiling of salt-stress tolerant proton gradient regulator 5 (PGR5) in Gossypium arboreum 1, 2 2 2 Muhammad Naveed SHAHID *, Adil JAMAL , Beenish AFTAB , Bahaeldeen Babiker MOHAMED , 1 2 2 2 Javed Iqbal WATTOO , Muhammad Sarfraz KIANI , Bushra RASHID , Tayyab HUSNAIN 1 Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan 2 Center of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan Received: 10.04.2015 Accepted/Published Online: 19.10.2015 Final Version: 21.06.2016 Abstract: Salinity is among the significant abiotic stresses adversely affecting plant development and yield for a wide range of crops. For quantitative and qualitative expression studies of proton gradient regulator 5 (PGR5) under salt stress, differential display RT-PCR and rapid amplification of cDNA ends (RACE) were performed on salt-stress responsive Gossypium arboreum. Alignment of genomic and cDNA sequences revealed that the GPGR5 gene comprises a single open reading frame of 96 amino acids and contains no introns. Alignment of cotton GPGR5 complete amino acid sequence with PGR5 from other plants revealed the following identities: Gossypium raimondii (97%), Amaranthus hybridus (72%), Vitis vinifera (69%), Medicago truncatula (68%), Cucumis melo (62%), Arabidopsis thaliana (62%), Portulaca oleracea (61%), Portulaca grandiflora (60%), and Zea mays (55%). The expression profile was studied in different plant tissues (stem, leaf, and root) under the abiotic stresses salt, drought, and cold. The results showed a 7-fold increased expression of GPGR5 in leaf tissue in salt stress and almost no induction of transcription in root and stem tissues in salt stress. This gene has a good expression pattern under cold stress compared to salt and

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