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Study of in vitro anther culture in selected genotypes of genus Capsicum

The combined effect of anther incubation time on CP induction medium (12, 14, and 16 days) and 2 concentrations of kinetin in R1 regeneration medium (0.1 and 0.3 mg/L) on the effectiveness of androgenesis was investigated in 17 genotypes of Capsicum grown in Poland. | Turkish Journal of Biology http://journals.tubitak.gov.tr/biology/ Research Article Turk J Biol (2014) 38: 118-124 © TÜBİTAK doi:10.3906/biy-1307-50 Study of in vitro anther culture in selected genotypes of genus Capsicum Dorota OLSZEWSKA, Anna KISIALA*, Aleksandra NIKLAS-NOWAK, Pawel NOWACZYK Department of Genetics, Physiology, and Plant Biotechnology, Faculty of Agriculture and Biotechnology, University of Technology and Life Sciences, Bydgoszcz, Poland Received: 22.07.2013 Accepted: 24.09.2013 Published Online: 02.01.2014 Printed: 24.01.2014 Abstract: The combined effect of anther incubation time on CP induction medium (12, 14, and 16 days) and 2 concentrations of kinetin in R1 regeneration medium (0.1 and 0.3 mg/L) on the effectiveness of androgenesis was investigated in 17 genotypes of Capsicum grown in Poland. Plant material consisted of breeding lines and intraspecific hybrids of C. annuum; the species of C. frutescens, C. chinense, and C. baccatum var. pendulum; interspecific hybrids F1 (C. frutescens × C. chinense) and F1 (C. frutescens × C. baccatum); and doubled haploid lines derived from the hybrids. The results of the tested variants of the experiment were compared with the androgenic response of the control anthers cultured according to the standard protocol previously developed for pepper (12 days incubation on CP, 0.1 mg/L kinetin in R1). Under control conditions, androgenic embryos regenerated only from anthers of 3 of the tested genotypes, whereas application of the selected modifications promoted embryo development in an additional 12 genotypes. The highest effectiveness of androgenesis was observed after 16 days of anther incubation on CP medium combined with 0.1 mg/L kinetin in R1 medium. Twelveand 14-day-long anther incubation was more effective when followed by transferring anthers onto R1 medium supplemented with 0.3 mg/L kinetin. Key words: Androgenesis, embryo induction, haploid, incubation length, kinetin, pepper 1. .