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Comparison of the volatiles of Daphne pontica L. and D. oleoides Schreber subsp. oleoides isolated by hydro- and microdistillation methods

Aerial parts of Daphne pontica were collected from Ilgaz-Çankırı, and D. oleoides subsp. oleoides was collected from 2 different localities (Ayrancı-Karaman and Ilgaz-Çankırı) in Turkey. The samples were subjected to hydrodistillation and microdistillation. | Turkish Journal of Biology Turk J Biol (2013) 37: 114-121 © TÜBİTAK doi:10.3906/biy-1204-20 http://journals.tubitak.gov.tr/biology/ Research Article Comparison of the volatiles of Daphne pontica L. and D. oleoides Schreber subsp. oleoides isolated by hydro- and microdistillation methods 1, 2 3 İlhan GÜRBÜZ *, Betül DEMİRCİ , Gerhard FRANZ , 4 5 2 Kemal Hüsnü Can BAŞER , Erdem YEŞİLADA , Fatih DEMİRCİ 1 Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06300 Ankara, Turkey 2 Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470 Eskişehir, Turkey 3 Department of Pharmaceutical Biology, Faculty of Pharmacy, Regensburg University, 93020 Regensburg, Germany 4 Department of Botany and Microbiology, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia 5 Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, 34755 Kayışdağı, İstanbul, Turkey Received: 10.04.2012 Accepted: 11.08.2012 Published Online: 10.01.2013 Printed: 01.02.2013 Abstract: Aerial parts of Daphne pontica were collected from Ilgaz-Çankırı, and D. oleoides subsp. oleoides was collected from 2 different localities (Ayrancı-Karaman and Ilgaz-Çankırı) in Turkey. The samples were subjected to hydrodistillation and microdistillation. The resulting volatile samples were analyzed both by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS), respectively. The main components for D. pontica were identified as hexahydrofarnesyl acetone (8.6%), carvacrol (8.5%), dihydroedulane II (4.7%), (E)-geranyl acetone (4.6%), and thymol (4.5%), while nonacosane (42.5% and 27.2%), hexadecanoic acid (24.4% and 20.0%), phytol (12.3%), and carvacrol (5.0%) were identified as the main components of D. oleoides subsp. oleoides obtained by hydrodistillation. Carvacrol (12.0%), thymol (7.7%), dihydroactinidiolide (7.2%), bicyclosesquiphellandrene (5.5%), and (Z)-3-hexenal (4.1%) were the major components in D. pontica, while carvacrol .