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Acid proteinase enzyme activity in Candida albicans strains: A comparison of spectrophotometry and plate methods

In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. | S. AKÇAĞLAR, B. ENER, O. TÖRE Turk J Biol 35 (2011) 559-567 © TÜBİTAK doi:10.3906/biy-1002-39 Acid proteinase enzyme activity in Candida albicans strains: a comparison of spectrophotometry and plate methods Sevim AKÇAĞLAR*, Beyza ENER, Okan TÖRE Uludağ University, Faculty of Medicine, Department of Microbiology and Infectious Diseases, 16059 Bursa - TURKEY Received: 26.02.2010 Abstract: In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates that, in terms of proteinase activity, there were no statistically significant differences between strains obtained from infection and colonization sites by either method (P > 0.05). Key words: Candida albicans, secreted aspartyl proteinase (SAP) activity, virulence Candida albicans suşlarında asit proteinaz enzim aktivitesi: spektrofotometri ve petri metodlarının karşılaştırılması Özet: Son yıllarda bütün dünyada fungal enfeksiyon olgularında artış gözlenmektedir. Patojenik funguslarla ilgili araştırmalar artmış olmasına rağmen, hala virulansla ilgili çalışmalara ihtiyaç vardır. Bu çalışmada kandan izole edilen Candida albicans suşlarında in vitro virulans faktörlerinin araştırılması amaçlanmıştır. Steril vücut sıvılarından izole edilen 30 C. albicans (15’i kolonizasyon, 15’i enfeksiyon) suşlarının proteinaz enzim aktivitesi spektrofotometrik ve petri plak yöntemi ile değerlendirilmiştir. İki yöntem .