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Brown alga Sargassum muticum inhibits proinflammatory cytokines, iNOS, and COX-2 expression in macrophage RAW 264.7 cells

The present study was designed to elucidate the pharmacological and biological effects of Sargassum muticum extracts on the production of inflammatory mediators in macrophages. S. muticum was extracted with 80% EtOH. The extract was then successively partitioned with n-hexane, CH2Cl2, EtOAc, BuOH, and water. | Turk J Biol 34 (2010) 25-34 © TÜBİTAK doi:10.3906/biy-0810-14 Brown alga Sargassum muticum inhibits proinflammatory cytokines, iNOS, and COX-2 expression in macrophage RAW 264.7 cells Weon-Jong YOON1,2, Young Min HAM1,2, Wook Jae LEE1,2, Nam Ho LEE2,3, Chang-Gu HYUN1,2,* 1Research Group for Cosmetic Materials, Jeju Biodiversity Research Institute (JBRI), Jeju High-Tech Development Institute (HiDI), Jeju 699-943 - KOREA 2Jeju Seaweed Industry Development Agency and 3Department of Chemistry, Cheju National University, Jeju 690-756 - KOREA Received: 16.10.2008 Abstract: The present study was designed to elucidate the pharmacological and biological effects of Sargassum muticum extracts on the production of inflammatory mediators in macrophages. S. muticum was extracted with 80% EtOH. The extract was then successively partitioned with n-hexane, CH2Cl2, EtOAc, BuOH, and water. The results indicate that the CH2Cl2 fraction of S. muticum extract was an effective inhibitor of LPS-induced NO and PGE2 production in RAW 264.7 cells. These inhibitory effects of the CH2Cl2 fraction of S. muticum included dose-dependent decreases in the expression of iNOS and COX-2 proteins and iNOS and COX-2 mRNA. To test the inhibitory effects of S. muticum fractions on other cytokines, we also measured IL-1β and IL-6 mRNA expression by RT-PCR in LPS-stimulated RAW 264.7 macrophage cells. In these assays, the CH2Cl2 fraction of S. muticum decreased the expression of IL-1β and IL-6 mRNA in a dose-dependent manner. Based on these results, we suggest that S. muticum extracts may be considered possible antiinflammatory candidates for human health. Key words: Algae, extract, inflammation, Sargassum muticum Kahverengi alg Sargassum muticum RAW 264,7 makrofaj hücrelerindeki iltihaba sebep olan sitokinler, iNOS ve COX-2 ekspresyonunu engeller Özet: Bu çalışma makrofajlardaki iltihap iyileştiricilerin üretimi üzerine Sargassum muticum özütünün farmakolojik ve biyolojik etkilerini aydınlatmak için