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Electrophoretic analysis of Candida albicans Isolates collected from Turkish hospitals by Nativepolyacrylamide gel electrophoresis (N-PAGE)

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Native-polyacrylamide gel electrophoresis (N-PAGE) was used to characterize Candida albicans strains isolated from vaginal specimens of patients suffering from vaginitis in Ankara hospitals, Turkey. | Turk J Biol 24 (2000) 803–808 © TÜBİTAK Electrophoretic Analysis of Candida albicans Isolates Collected From Turkish Hospitals by NativePolyacrylamide Gel Electrophoresis (N-PAGE) Özlem OSMANAĞAOĞLU Gazi University, Faculty of Arts and Sciences, Department of Biology, Ankara-TURKEY Sefa C. SAÇILIK Ankara University, Faculty of Sciences, Department of Biology, Ankara-TURKEY Ufuk GÜNDÜZ Middle East Technical University, Faculty of Arts and Sciences, Department of Biology, Ankara-TURKEY Nurten ALTANLAR Ankara University, Faculty of Pharmacy, Department of Microbiology, Ankara-TURKEY Cumhur ÇÖKMÜŞ Ankara University, Faculty of Sciences, Department of Biology, Ankara-TURKEY Received: 04.08.1999 Abstract: Native-polyacrylamide gel electrophoresis (N-PAGE) was used to characterize Candida albicans strains isolated from vaginal specimens of patients suffering from vaginitis in Ankara hospitals, Turkey. Three different protein band profiles were observed among these 38 C. albicans isolates when whole cell protein extracts of these strains were used in N-PAGE. 34 of the 38 isolates were shown to belong to a single group or clone with a dissemination percentage of 89, while three isolates belonged to another group with a percentage of 8. Furthermore only one C. albicans isolate was detected with a dissemination percentage of 3 and assigned to a third group. The results presented here also suggested that N-PAGE may be an efficacious method for differentiating between C. albicans strains. Türk Hastanelerinden Toplanmış Olan Candida albicans İzolatlarının Native-Poliakrilamid Jel Elektroforez ile Elektroforetik Analizi Özet: Ankara’daki hastanelerde vajinit problemli hastalardan toplanmış olan 38 adet Candida albicans suşu native-poliakrilamid jel eletroforez (N-PAGE) ile karakterize edilmiştir. C. albicans izolatlarının hücre protein ekstraktları N-PAGE’de kullanıldığı zaman üç değişik protein bant patternine rastlanmıştır. Bu çalışmanın sonucunda toplam 38 C. albicans