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Physiological studies on the plant cell wall degrading enzymes of the rumen bacterium Ruminococcus flavefaciens

Two forms of Ruminococcus flavefaciens, the wild type and a trained culture of strain 17 were compared for their cellulose and xylan degradative ability when then were grown in ryegrass, cellobiose and xylan for different incubation times. | Turk J Biol 25 (2001) 113-122 © TÜB‹TAK Physiological Studies on the Plant Cell Wall Degrading Enzymes of the Rumen Bacterium Ruminococcus flavefaciens Emin ÖZKÖSE IGER Research Institute, Plas Gogerddan, Aberystwyth, SY23 3EB, UK M. Sait EK‹NC‹ Kahramanmarafl Sütçü ‹mam University, Faculty of Agriculture, Department of Animal Science, Kahramanmarafl-TURKEY Numan ÖZCAN Çukurova Universit, Faculty of Agriculture, Department of Animal Science, Adana-TURKEY Received: 03.09.1999 Abstract: Two forms of Ruminococcus flavefaciens, the wild type and a trained culture of strain 17 were compared for their cellulose and xylan degradative ability when then were grown in ryegrass, cellobiose and xylan for different incubation times. No cell-associated corboxymethylcellulase (CMCase) activity was observed after 1-day incubation in cellobiose, as no supernatant activity was detected after 1 and 3 days incubation in xylan and 2 days incubation in cellobiose grown culture. Both CMCase and xylanase activities of trained culture were higher than those of wild type culture when they were grown in ryegrass. Wild type cultures, however, showed higher levels of both activities when grown in medium containing xylan. Key Words: Ruminococcus flavefaciens, cellulase, xylanase. Bitki Hücre Duvar›n› Parçalayan Rumen Bakterisi Ruminococcus flavefaciens’e Ait Enzimler Üzerine Fizyolojik Bir Çal›flma Özet: R. flavefaciens’in iki formu; yabani form ile ksilene karfl› e¤itilmifl form, farkl› inkübasyon zamanlar›nda ve enerji kayna¤› olarak cavdar saman›, sellobioz, ve ksilen içeren besi yerlerinde yetifltirilerek selulozu ve ksileni parçalama kabiliyetleri karfl›laflt›r›lm›flt›r. Sellobioz üzerinde 1 gün inkübasyondan sonra hücresel; ksilen üzerinde 1 ve 3 gün inkübasyondan sonra ve sellobioz üzerinde 2 gün inkübasyondan sonra supernatant karboksimetilsellulaz (CMCaz) aktivitesi gözlenmemifltir. E¤itilmifl formun çavdar saman› üzerindeki CMCaz ve ksilenaz etkinlikleri yabani formdan .