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Assessment of resistance gene analog, peroxidase, and WRKY gene polymorphisms in the genus Lens Miller

Resistance gene analogs (RGAs), peroxidases (POXs), and WRKY transcription factors are gene families related to stressresponse pathways that regulate enhancement of stress tolerance in crop plants. In this study, the polymorphisms of these gene families were determined in the genus Lens Miller and Vicia montbretii (Fish. & Mey) Davis and Plitmann. | Turkish Journal of Botany Turk J Bot (2016) 40: 121-129 © TÜBİTAK doi:10.3906/bot-1501-41 http://journals.tubitak.gov.tr/botany/ Research Article Assessment of resistance gene analog, peroxidase, and WRKY gene polymorphisms in the genus Lens Miller 1 2 1, Duygu SARI , Nedim MUTLU , Cengiz TOKER * Department of Field Crops, Faculty of Agriculture, Akdeniz University, Antalya, Turkey 2 Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, Antalya, Turkey 1 Received: 20.01.2015 Accepted/Published Online: 01.07.2015 Final Version: 09.02.2016 Abstract: Resistance gene analogs (RGAs), peroxidases (POXs), and WRKY transcription factors are gene families related to stressresponse pathways that regulate enhancement of stress tolerance in crop plants. In this study, the polymorphisms of these gene families were determined in the genus Lens Miller and Vicia montbretii (Fish. & Mey) Davis and Plitmann. A total of 32 genotypes of 7 lentil taxa, including 1 accession of cultivated lentil (Lens culinaris Medik. subsp. culinaris), 11 accessions of L. culinaris subsp. orientalis (Boiss.) Ponert, 3 accessions of L. culinaris subsp. tomentosus Ladiz., 4 accessions of L. culinaris subsp. odemensis Ladiz., 3 accessions of L. ervoides (Brign.) Grande, 4 accessions of L. lamottei Czefr., 4 accessions of L. nigricans (M.Bieb.) Godr., and 2 accessions of V. montbretii, were used. Sixty-eight RGA, 22 POX, and 13 WRKY primers were used for molecular analysis. After using these degenerate primers in polymerase chain reaction, the highest polymorphism rate (90%) was observed in WRKY, followed by POX (89%) and RGA (79%). Lens culinaris Medik. subsp. culinaris had the lowest number of RGA and POX bands. Most RGA markers were obtained from L. culinaris subsp. tomentosus, L. culinaris subsp. orientalis, L. culinaris subsp. odemensis, L. ervoides, and L. nigricans. Similarly, the number of WRKY markers was the highest in L. nigricans and L. culinaris .