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Localization and distribution of nickel and other elements in in-vitro grown Alyssum corsicum exhibiting morphological changes in trichomes: initial insights into molecular mechanisms of nickel hyperaccumulation

Alyssum corsicum Duby is a candidate plant used for phytomining and phytoremediation studies with its high Ni-accumulating ability of the aboveground tissues. Metal localization and concentration by hyperaccumulator plants and the physiological basis of these phenomena have been of great interest in recent years. A. corsicum seeds were grown at different concentrations of Ni in Murashige and Skoog medium. | Turkish Journal of Botany Turk J Bot (2013) 37: 1115-1124 © TÜBİTAK doi:10.3906/bot-1211-47 http://journals.tubitak.gov.tr/botany/ Research Article Localization and distribution of nickel and other elements in in-vitro grown Alyssum corsicum exhibiting morphological changes in trichomes: initial insights into molecular mechanisms of nickel hyperaccumulation 1 1, 2 1 Seniha Selcen BABAOĞLU AYDAŞ , Leyla AÇIK *, Danika LEDUC , Nezaket ADIGÜZEL , 3 1 4 Şeküre Şebnem ELLİALTIOĞLU , Zekiye SULUDERE , Yusuf Kağan KADIOĞLU 1 Department of Biology, Faculty of Science, Gazi University, Teknikokullar, Ankara, Turkey 2 Department of Chemistry & Biochemistry, College of Science, California State University, East Bay, Hayward, California, USA 3 Agricultural Faculty, Ankara University, Dışkapı, Ankara, Turkey 4 Department of Geological Engineering, Faculty of Engineering, Ankara University, Tandoğan, Ankara, Turkey Received: 22.11.2012 Accepted: 21.07.2013 Published Online: 30.10.2013 Printed: 25.11.2013 Abstract: Alyssum corsicum Duby is a candidate plant used for phytomining and phytoremediation studies with its high Ni-accumulating ability of the aboveground tissues. Metal localization and concentration by hyperaccumulator plants and the physiological basis of these phenomena have been of great interest in recent years. A. corsicum seeds were grown at different concentrations of Ni in Murashige and Skoog medium. Multielement concentrations of the plant roots and shoots were determined by polarized energy dispersive X-ray fluorescence. Ni, Ca, Mg, and Fe localization and concentration at the upper and lower leaf surfaces were determined by scanning electron microscopy coupled with the energy-dispersive X-ray analysis technique (SEM-EDX). Results showed that Ni is accumulated more by the shoots than the roots of the plant and that Ni is concentrated mainly in the trichome and the stoma guard cells of the leaves. Trichome density on the leaves was reduced, and some .