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Antioxidant, anti-inflammatory and hepatoprotective activities on Carbon Tetrachloride - induced hepatic damage in mice of Ixora Duffii leaf extract

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The in vitro total antioxidant and anti-inflammatory capacity of the methanol extract of Ixora duffii leaf (―Trang to‖ in Vietnamese) was investigated using the phosphomolypdenum method and the bovine serum albumin denaturation test, respectively. The results showed that the methanol extract of I. duffii leaf possessed relatively high antioxidant activity (OD0,5 = 15.551 ± 0.344 μg/mL) compared to that of the standard, trolox (OD0,5=2.315 ± 0.083 μg/mL). The antiinflammatory effect using bovine serum albumin denaturation test showed that the activity of the methanol extract of I. duffii leaf (EC50= 6.03 ± 0.12 μg/mL) was comparable to that of the reference drug, diclofenac sodium (EC50=0.57 ± 0.21 µg/mL). To evaluate the hepatoprotective activity of the methanol extract of I. duffii leaf, the liver damage was induced in mice given with carbon tetrachloride (CCl4) mixed in olive oil (1:4) at a dose of 2.5 mL/kg body weight/day. As a control, the standard hepatoprotective agent, silymarin, was used at the dose of 16 mg/kg body weight for 4 consecutive weeks. I. duffii leaf extract was given at three different doses 100, 200 and 400 mg/kg body weight. The results showed the effective dose-dependent reduction of transaminase enzyme (ALT and AST) levels in sera. In addition, I. duffii leaf extract also improved the oxidative stress status of the liver through effective reduction of MDA level and increase of GSH level in the liver. Histopathological examination of the liver tissue revealed that the mice treated with I. duffii leaf extract showed significant improvement of liver tissue damages similar to silymarin treatment compared to the non-treated control group. The present results demonstrated that I. duffii leaf extract has potent antioxidant and hepatoprotective activity. | Antioxidant, anti-inflammatory and hepatoprotective activities on Carbon Tetrachloride - induced hepatic damage in mice of Ixora Duffii leaf extract