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Sap transmission and detection of sunflower necrosis virus in sunflower

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The present study was carried out to know the sap transmission nature of sunflower necrosis virus and detection by serological and molecular means. The disease was successfully transmitted through mechanical sap using 0.05 M potassium phosphate buffer. A maximum transmission of 52.33 per cent on cultivar KBSH-44, 51.33 per cent on KBSH-1, and 48.33 per cent on Morden. Under artificial inoculation, the newly emerged leaves showed slight downward curling, puckering and chlorosis followed by necrosis. The sunflower genotypes subjected for serological detection by using DAC-ELISA a positive reaction with Tobacco Streak Virus (TSV) antisera was obtained, further, The RT-PCR was performed using RKJ primers specific for the coat protein gene of TSV and resulted in an amplicon of approximately 700bp. These results thus prove the transmissible nature of SNV through mechanical sap. | Sap transmission and detection of sunflower necrosis virus in sunflower

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