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Electrophoretic profile of seminal plasma proteins of specific fractions of large white Yorkshire boar semen

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The objective of the research was to study the protein content and electrophoretic profile of specific fractions of Large White Yorkshire (LWY) boar semen. Sperm rich fractions (SRF) of semen were collected from four sexually mature LWY boars as fractions of 10 mL each, by gloved hand method. For analysis, the first 10 mL of the SRF, rest of the SRF and sperm free fraction (SFF) were collected separately. The protein content of the semen fractions was assessed and the proteins were subjected to one dimensional discontinuous sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to assess the electrophoretic profile. A protein content of 20.71 ± 2.63 mg/mL was observed in the first 10 mL of SRF, 21.77 ± 1.84 mg/mL in the remaining SRF and 20.52 ± 2.06 mg/mL in the SFF. An average number of 13.5 ± 0.51 bands were observed in first 10 mL of SRF, 12.92 ± 0.44 bands in the remaining part of SRF and 12.78 ± 0.23 bands in the SFF. The proteins observed varied in the molecular weight from 18 - 244 kDa, 8 – 242 kDa and 9 – 248 kDa in first 10 mL of SRF, remaining SRF and SFF, respectively. | Electrophoretic profile of seminal plasma proteins of specific fractions of large white Yorkshire boar semen