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Differential DNA methylation under drought stress in maize

DNA methylation plays a vital roles in plant response to drought stress. Therefore, the present study was carried out to investigate differential cytosines methylation in leaves of maize under drought stress by using AMP method. Thirty days old maize seedlings were subjected to water deficit for nine days and the leaves of drought stressed plants and control plants were collected and stored at -20 until DNA extraction and subsequence DNA analysis. We isolated and sequenced 18 differentially methylated fragments. DMF 4, DMF 10, and DMF 13 were found to be homologous to lncRNA genes, DMF3 is similar to protein dehydrationinduced 19 homologue 3 and DMF 18 is homologous to nudix hydrolase 2 gene. The obtained results indicated that lncRNAs are consider as important regulators in response of maize plant to drought stress.