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Bioremediation of recalcitrant textile azo dye - Methyl orange by bacillus subtilis BRTSI-3 Isolated from textile effluents

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Textile effluent samples collected from dye contaminated area of Ranipet, Vellore have been used for isolating bacterial strains. Among various bacterial isolates, BRTSI-3 was selected and was further characterized using morphological and biochemical analysis. 16S rDNA sequencing confirmed the strain BRTSI3 as Bacillus subtilis (NCBI accession number MH412808). The culture conditions for maximizing bacterial biomass were found to be optimized at 35oC and pH 8.0. Bacillus subtilis effectively decolorized methyl orange in nutrient broth within 48 h of incubation. | Bioremediation of recalcitrant textile azo dye - Methyl orange by bacillus subtilis BRTSI-3 Isolated from textile effluents