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Carotenoid determination in recent marine sediments - practical problems during sample preparation and HPLC analysis

An analytical procedure for the analysis of carotenoids in marine sediments rich in organic matter has been developed. Analysis of these compounds is difficult; the application of methods used by other authors required optimization for the samples studied here. | Carotenoid determination in recent marine sediments - practical problems during sample preparation and HPLC analysis Current Chemistry Letters 6 2017 91 104 Contents lists available at GrowingScience Current Chemistry Letters homepage www.GrowingScience.com Carotenoid determination in recent marine sediments - practical problems during sample preparation and HPLC analysis Magdalena Krajewska Małgorzata Szymczak-Żyła and Grażyna Kowalewska Marine Pollution Laboratory Institute of Oceanology Polish Academy of Sciences Powstańców Warszawy 55 81-712 Sopot Poland CHRONICLE ABSTRACT Article history An analytical procedure for the analysis of carotenoids in marine sediments rich in organic Received January 2 2017 matter has been developed. Analysis of these compounds is difficult the application of Received in revised form methods used by other authors required optimization for the samples studied here. The March 1 2017 analytical procedure involved multiple ultrasound-assisted extraction with acetone followed by Accepted April 21 2017 liquid-liquid extraction acetone extract benzene water - 15 1 10 v v v and HPLC analysis. Available online The influence of column temperature on pigment separation and the quantification method April 22 2017 were investigated a temperature of 5 C was selected for the Lichrospher 100 RP-18e column. Keywords The pigments in the sediment extract were quantified using a method based on HPLC analysis Carotenoids Sediments at 450 nm and spectrophotometric measurements at 450 nm and extinction coefficients Analysis were determined for standard solutions at this wavelength. It is very important to use the value Extraction of the extinction coefficient appropriate to the wavelength at which the detection of carotenoids HPLC was carried out. 2017 Growing Science Ltd. All rights reserved. 1. Introduction Carotenoids are a large group of natural compounds at present ca 700 are known ubiquitous in the aquatic environment.1 They occur mainly in .