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Effect of vitamin on accumulation of PHB by Zobellella species under submerged fermentation process

In the current context of eco-pollution, the substitute of conventional plastics with bioplastics is a big confront. On account of that, a non-growth associated PHB production study was conducted using Zobellella species under submerged fermentation process. As a result, Zobellella species showed the ability to accumulate 2.2g/L of PHB at pH 8. 0, temperature 37oC, salt concentration 2% in modified growth medium containing vitamins. Then, primary structural characterization of extracted polymer was conducted by FTIR analysis. The high intense peak was obtained at 1720.50 cm-1 corresponding to ester carbonyl (C=O) stretching vibration of PHB. This finding will be paved the way of researchers during selection of suitable vitamins containing inexpensive carbon sources for cost effective PHB production. Thus, extensive research is highly indispensible for bioconversion of vitamin containing raw materials to PHB. | Effect of vitamin on accumulation of PHB by Zobellella species under submerged fermentation process Int.J.Curr.Microbiol.App.Sci 2017 6 2 1310-1316 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 6 Number 2 2017 pp. 1310-1316 Journal homepage http www.ijcmas.com Original Research Article http dx.doi.org 10.20546 ijcmas.2017.602.148 Effect of Vitamin on Accumulation of PHB by Zobellella Species under Submerged Fermentation Process S. Maity1 S. Das2 and D.P. Samantaray1 1 Department of Microbiology CBSH OUAT Bhubaneswar-3 Odisha-751003 India 2 Department of Life Science LEnME NIT Rourkela Odisha - 769008 India Corresponding author ABSTRACT Keywords In the current context of eco-pollution the substitute of conventional plastics with bioplastics is a big confront. On account of that a non-growth associated PHB production Eco-pollution study was conducted using Zobellella species under submerged fermentation process. As a PHB Zobellella result Zobellella species showed the ability to accumulate 2.2g L of PHB at pH 8. 0 FTIR Vitamins. temperature 37oC salt concentration 2 in modified growth medium containing vitamins. Then primary structural characterization of extracted polymer was conducted by FTIR Article Info analysis. The high intense peak was obtained at 1720.50 cm-1 corresponding to ester Accepted carbonyl C O stretching vibration of PHB. This finding will be paved the way of 22 January 2017 researchers during selection of suitable vitamins containing inexpensive carbon sources for Available Online cost effective PHB production. Thus extensive research is highly indispensible for 10 February 2017 bioconversion of vitamin containing raw materials to PHB. Introduction In response to risk associated with found in Gram positive Kalaivani and petrochemical based plastic waste and its Sukumaran 2015 Mohapatra et al. 2016 and effect on the environment there has been Chaudhry et al. 2010 and Gram negative considerable interest on