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Comparison and demonstration of surface keratin staining quality by modified Papanicalou stain, modified mallory’s stain and hematoxylin and eosin stain in verrucous carcinoma and normal keratinizing epithelium

Observation of normal or abnormal keratin forms is the major criteria in histopathological diagnosis of Verrucous carcinoma. The aim of the study is to evaluate and compare the distinct staining and surface keratin in verrucous carcinoma and normal keratinizing epithelium by routine hematoxylin and eosin (H&E) stain, modified papanicolou stain and modified Mallory’s stain. The known cases of verrucous carcinoma and tissues from normal keratinizing epithelial regions with sufficient paraffin embedded tissue were selected. | Comparison and demonstration of surface keratin staining quality by modified Papanicalou stain modified mallory s stain and hematoxylin and eosin stain in verrucous carcinoma and normal keratinizing epithelium Int.J.Curr.Microbiol.App.Sci 2017 6 2 1287-1293 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 6 Number 2 2017 pp. 1287-1293 Journal homepage http www.ijcmas.com Original Research Article http dx.doi.org 10.20546 ijcmas.2017.602.145 Comparison and Demonstration of Surface Keratin Staining Quality by Modified Papanicalou Stain Modified Mallory s Stain and Hematoxylin and Eosin Stain in Verrucous Carcinoma and Normal Keratinizing Epithelium Santhosh Kumar Caliaperoumal1 Sudhakar Srinivasan2 and Meer Ahmed Ibrahim Munshi3 1 Department of Dentistry Vinayaka Mission s Medical College and Hospital Karaikal Puducherry India 2 Department of Dentistry Karpagam Medical College and Hospital Coimbatore India 3 Department of Oral surgery Sri Ramakrishna Dental College and Hospital Coimbatore India Corresponding author ABSTRACT Keywords Observation of normal or abnormal keratin forms is the major criteria in histopathological diagnosis of Verrucous carcinoma. The aim of the study is to evaluate and compare the Verrucous distinct staining and surface keratin in verrucous carcinoma and normal keratinizing carcinoma VC epithelium by routine hematoxylin and eosin H amp E stain modified papanicolou stain and Modified modified Mallory s stain. The known cases of verrucous carcinoma and tissues from Papanicolou MP stain Modified normal keratinizing epithelial regions with sufficient paraffin embedded tissue were Mallory s MM selected. The three sections from each block were stained with H amp E stain modified stain Hematoxylin Papanicolou stain and modified Mallory s stain. The modified Mallory s stain modified and Eosin H amp E Papanicolou stain distinctively and clearly stained surface keratin compared to H amp E stain. stain. The .