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Bioassay studies for testing toxicity of novel insecticides against Spodoptera litura (Fabricius.)

Bioassay studies were conducted in the Department of Entomology, College of Agriculture, Rajendranagar, Hyderabad to determine the relative toxicity of selected novel insecticides viz., chlorfluazuron, chlorantraniliprole, spinetoram, indoxacarb, emamectin benzoate and spinosad against second instar larvae ofS. Litura by topical application, residue method and surface diet method. The data on the toxicity of insecticides to S. litura, clearly indicated that among the six insecticides tested against the second instar, emamectin benzoate, spinetoram, indoxacarb, chlorfluazuron, chlorantraniliprole showed greater toxicity against S. litura compared to spinosad in all the three methods of application. | Int.J.Curr.Microbiol.App.Sci 2020 9 7 1609-1613 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 7 2020 Journal homepage http www.ijcmas.com Original Research Article https doi.org 10.20546 ijcmas.2020.907.185 Bioassay Studies for Testing Toxicity of Novel Insecticides against Spodoptera litura Fabricius. Sashanka Sekhar Dash M. Lava Kumar Reddy G. Sridevi Bharathi N. Bhat Jayashankar Telangana State Agricultural University Hyderabad-500030 Telangana India Corresponding author ABSTRACT Keywords Bioassay studies were conducted in the Department of Entomology College of Insecticides Agriculture Rajendranagar Hyderabad to determine the relative toxicity of Spodoptera litura selected novel insecticides viz. chlorfluazuron chlorantraniliprole spinetoram Fabricius indoxacarb emamectin benzoate and spinosad against second instar larvae ofS. Bioassay Studies Litura by topical application residue method and surface diet method. The Article Info data on the toxicity of insecticides to S. litura clearly indicated that among the six insecticides tested against the second instar emamectin benzoate Accepted 14 June 2020 spinetoram indoxacarb chlorfluazuron chlorantraniliprole showed greater Available Online toxicity against S. litura compared to spinosad in all the three methods of 10 July 2020 application. Introduction conditions. Also such baseline data would provide a record for detecting resistance level Bioassay is defined as the measurement of of the insect pests to various insecticides at the potency of any stimulus which may be different periods. physical chemical biological physiological or psychological etc. by means of the The tobacco caterpillar Spodopteralitura reactions which it produce in the living Fabricius Lepidoptera Noctuidae is also organism Finney 1952 . Bioassay helps in an important polyphagous pest infesting ascertaining the potency and relative toxicity crops of major economic importance. It was of .