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Determining the prevalence of Helicobacter pylori infection by molecular identification of 16S rRNA in gastric patients in Hai Duong provincial general hospital

In this study, 180 gastric biopsies were taken from patients of Hai Duong Provincial General Hospital who had come to the hospital to be treated for gastritis during the period January 2015 to July 2015. All of the subjects were 18-80 years old; they had chronic gastritis, a stomach ulcer or gastric cancer; they did not use antibiotics for 30 days prior to our endoscopy. | JOURNAL OF SCIENCE OF HNUE DOI 10.18173 2354-1059.2015-00091 Chemical and Biological Sci. 2015 Vol. 60 No. 9 pp. 148-153 This paper is available online at http stdb.hnue.edu.vn DETERMINING THE PREVALENCE OF Helicobacter pylori INFECTION BY MOLECULAR IDENTIFICATION OF 16S rRNA IN GASTRIC PATIENTS IN HAI DUONG PROVINCIAL GENERAL HOSPITAL Le Thi Phuong Hai Duong Medical Technical University Abstract. In this study 180 gastric biopsies were taken from patients of Hai Duong Provincial General Hospital who had come to the hospital to be treated for gastritis during the period January 2015 to July 2015. All of the subjects were i 18-80 years old ii they had chronic gastritis a stomach ulcer or gastric cancer iii they did not use antibiotics for 30 days prior to our endoscopy. Endoscopic biopsy samples were taken from the patients at the antrum body and edge of the gastric ulcer organizations suspected of infiltrating stomach cancer. These DNA gastric biopsies were extracted amplified the 16S rRNA gene was sequenced and the results were compared in Genbank. The results showed that 80 of the 180 44.44 gastric patients were Helicobacter pylori positive. Keywords Gastritis Helicobacter pylori infection molecular identification 16S rRNA 1. Introduction Helicobacter pylori H. pylori is thought to be a common cause or culprit of chronic gastritis and peptic ulceration and it is considered to be the major risk factor for gastric cancer particularly in developing countries 1 2 . Many studies have shown that H. pylori is an important factor in precancer and gastric cancer 3 . In Vietnam a diagnosis of H. pylori presence in a patient s stomach is based on endoscopy images combined with the finding of H. pylori in a digestive juice culture and testing Urease for the presence of H. pylori 4 . Because culturing H. pylori from gastric biopsy specimens is a lengthy process and new super bacteria non-H. pylori are in existence analysis is difficult. It s necessary to apply modern .