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Optimization of total saponin extraction from tam that roots using the desirablity methodology

In Vietnam, Tam that hoang (Panax stipuleanatus Tsai et Feng) belonging to Araliaceous family is a valuable medicinal plant that possesses many biologically active compounds. Among them, flavonoids, polysaccharides, and saponins are the major constituents of this plant. | JOURNAL OF SCIENCE OF HNUE DOI 10.18173 2354-1059.2016-0052 Natural Sci. 2016 Vol. 61 No. 9 pp. 26-33 This paper is available online at http stdb.hnue.edu.vn OPTIMIZATION OF TOTAL SAPONIN EXTRACTION FROM TAM THAT ROOTS USING THE DESIRABLITY METHODOLOGY Nguyen Quang Tuyen and Dang Ngoc Quang Faculty of Chemistry Hanoi National University of Education Abstract In Vietnam Tam that hoang Panax stipuleanatus Tsai et Feng belonging to Araliaceous family is a valuable medicinal plant that possesses many biologically active compounds. Among them flavonoids polysaccharides and saponins are the major constituents of this plant. They have anti-oxidant anti-microbial anti-cancer and tonic properties. In this paper we studied the factors that affect the process of total saponin extraction from Tam that roots originated from Sapa town Lao Cai province. The total saponin extraction from Tam that roots was optimized using the desirability methodology with the experimental order of Box-Behnken design. The concentrations of ethanol the ratio of ethanol material v m the time of ultrasonic extraction with the frequency of 33 - 40 kHz are the three main considered factors. The optimized condition for total saponin extraction was found as follows ethanol concentration 65 ethanol material ratio 55 1 v m and extraction time 75 minutes. In this condition the maximum total saponin content was predicted to be 15.01 . Keywords Extraction saponin Panax stipuleanatus. 1. Introduction Extraction is the first step of great importance for the recovery and purification of active ingredients from plant materials. Traditional extraction techniques usually require many extraction hours and have low efficiency. Recently there have been numerous reports on the application of high intensity or power ultrasound in the extraction of various phyto-chemicals such as alkaloids flavonoids poly-saccharides proteins and essential oils from various parts of plants and their seeds 1-3 . The ultrasound-assisted .