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Synthesis and transfer of RNAi construct for potential knock-down of gene expression of root-knot nematode (Meloidogyne graminicola) to rice (Oryza sativa L.)

In this study, the Mgra16281 gene (ID: MK322955.1) enco ding an effector with the unknown function was cloned from the rice ro ot-knot nematode Meloidogyne graminicola isolated in Long An province. To knock-down the expression of this gene, an artificial microRNA was synthesized based on the Osa-MIR528 precursor and inserted into an expression vector. | 36 Trường Đại học Nông Lâm TP. Hồ Chí Minh Synthesis and transfer of RNAi construct for potential knock-down of gene expression of root-knot nematode Meloidogyne graminicola to rice Oryza sativa L. Phong V. Nguyen Phuong T. Nguyen Nhi T. Y. Le amp Loan T. N. Nguyen Department of Biotechnology Nong Lam University Ho Chi Minh City Vietnam ARTICLE INFO ABSTRACT Research Paper Effectors play key roles in the parasitism of the plant-parasitic nematode. Silencing the effector-coding genes was applied to study the Received April 01 2020 function and role of nematode effectors. In this study the Mgra16281 Revised May 28 2020 gene ID MK322955.1 encoding an effector with the unknown Accepted July 01 2020 function was cloned from the rice root-knot nematode Meloidogyne graminicola isolated in Long An province. To knock-down the ex- Keywords pression of this gene an artificial microRNA was synthesized based on the Osa-MIR528 precursor and inserted into an expression vector. Agrobacterium This microRNA can be expressed in rice to investigate the function of MGRA16281 of root-knot nematode via host-induced gene silencing Effector approach HIGS . Gene silencing MicroRNA Rice root-knot nematode Corresponding author Nguyen Vu Phong Email nvphong@hcmuaf.edu.vn Cited as Nguyen P. V. Nguyen P. T. Le N. T. Y. amp Nguyen L. T. N. 2020 . Synthesis and transfer of RNAi construct for potential knock-down of gene expression of root-knot nematode Meloidogyne graminicola to rice Oryza sativa L. . The Journal of Agriculture and Development 19 4 36-44. Tạp chí Nông nghiệp và Phát triển 19 4 www.jad.hcmuaf.edu.vn Trường Đại học Nông Lâm TP. Hồ Chí Minh 37 Tổng hợp và chuyển cấu trúc RNAi có khả năng bất hoạt gene tuyến trùng sưng rễ Meloidogyne graminicola vào cây lúa Oryza sativa L. Nguyễn Vũ Phong Nguyễn Thế Phương Lê Thị Yến Nhi amp Nguyễn Thị Ngọc Loan Bộ Môn Công Nghệ Sinh Học Trường Đại Học Nông Lâm TP.HCM TP. Hồ Chí Minh THÔNG TIN BÀI BÁO TÓM TẮT Bài báo khoa học Các effector có vai trò