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Summary of Biotechnology doctoral thesis: Study on development of DNA barcodes and research on in vitro micropropagation of Paramignya trimera

Thesis objectives: Evaluating the distribution of morphological characteristics, building a distribution map of the population of P. trimera and some species of the genus Paramignya; determining the genetic relationship of the P. trimera population by SSR markers; identification of DNA barcodes for identification of P. trimera; developed the protocol of in vitro micropropagation of P. trimera collected in Khanh Hoa, Vietnam. | MINISTRY OF EDUCATION AND VIETNAM ACADEMY TRAINING OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY SCIENCE AND TECHNOLOGY - Phi Thi Cam Mien Study on development of DNA barcodes and research on in vitro micropropagation of Paramignya trimera Specialized Biotechnology Code number 9.42.02.01 SUMMARY OF BIOTECHNOLOGY DOCTORAL THESIS Ha Noi 2021 The thesis was completed at Department Graduate University Science and Technology Institute of Biotechnology Supervisor Assoc.Prof. Dr Chu Hoang Ha Reviewer 1 Reviewer 2 Reviewer 3 . The thesis will be defended at . . Time . The thesis could be found at National Library of VietNam Library of Graduate University Science and Technology Institute of Biotechnology 1 INTRODUCTION 1. Introduction The Paramignya trimera Oliv. Guill belonging to the family Rutaceae distributed mainly in Khanh Hoa and Ninh Thuan provinces. According to published documents Vietnam had 7 species of the genus Paramignya. P. trimera is a small woody creeping wild tree distributed in areas over 200 meters where has arid climate thin topsoil. It has been widely used in traditional and folk medicine as an herbal plant for treating the liver diseases blood pressure and cancer. Recently many studies have shown that the root of P. trimera contains many alkaloids saponins courmarins and triterpenoids which have effects on acute hepatitis and inhibited some cancer cell lines. In particular P.trimera extract is so safe for use. Because of the highly effects of the P. trimera root for treating some cancer cell lines the price of this root is very high so its over exploited exhaustion. In addition due to the high ability of cross-pollination and highly frequency mutations combined with the phenomenon of asexual reproduction and polyploidy the phenotype of P.trimera in nature is very diverse with many similar characteristics with other plants in the genus Paramignya. The existence of such hybrids makes them difficult to distinguish and confusing based on only .