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A simple automated microplate method for determining reducing sugars in food extracts and synthetic serum using cupric-neocuproine as reductant

In the present work, a simple automated microplate method based on cupric ion reduction is described for determining total reducing sugars in food extracts and synthetic serum. The reaction of Cu(II)-Nc (cupric-neocuproine) with reducing sugars was performed in alkaline medium in microplates, and the absorbance of the formed highly colored Cu(I)-Nc chelate in a plate reader at 450 nm was recorded. The proposed method was applied to reducing sugars (glucose, fructose, galactose, maltose, and lactose) and their linear calibration curves were constructed. The detection and quantification limits (LOD and LOQ) for glucose were 0.14 and 0.46 µM, respectively.In the present work, a simple automated microplate method based on cupric ion reduction is described for determining total reducing sugars in food extracts and synthetic serum. The reaction of Cu(II)-Nc (cupric-neocuproine) with reducing sugars was performed in alkaline medium in microplates, and the absorbance of the formed highly colored Cu(I)-Nc chelate in a plate reader at 450 nm was recorded. The proposed method was applied to reducing sugars (glucose, fructose, galactose, maltose, and lactose) and their linear calibration curves were constructed. The detection and quantification limits (LOD and LOQ) for glucose were 0.14 and 0.46 µM, respectively. |