Đang chuẩn bị liên kết để tải về tài liệu:
Purification, refolding, and characterization of recombinant human paraoxonase-1

Không đóng trình duyệt đến khi xuất hiện nút TẢI XUỐNG Tải xuống

A high density lipoprotein (HDL)-linked enzyme with antioxidant and antiatherogenic properties, paraoxonase1(PON1), prevents the formation of atherosclerotic lesions in humans. In the present study, a recombinant hPON1 gene was produced using a small ubiquitin-related modifier (SUMO) fusion protein expression system. To that end, the hPON1 gene was amplified from human liver-ready cDNA, cloned into the expression vector pET SUMO, and expressed in Escherichia coli BL21 (DE3). |