Đang chuẩn bị liên kết để tải về tài liệu:
Handbook of Microbiological Media, Fourth Edition part 7

Không đóng trình duyệt đến khi xuất hiện nút TẢI XUỐNG Tải xuống

Handbook of Microbiological Media, Fourth Edition part 7 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Actinobacillus lignieresii Medium 55 Plant hydrolysate.5.0g Yeast extract.4.0g KH2PO4.0.55g KCl.0.425g CaCl2-2H2O.0.125g MgSO4-7H2O.0.125g Bromocresol Green.22.0mg Actidione cycloheximide .10.0mg MnSO4-4H2O.2.5mg FeCl3.2.5mg pH 5.5 0.2 at 25 C Source This medium is available as a premixed powder from Hi-Media. Caution Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the enumeration and detection of bacteria in specimens containing large numbers of yeasts and molds. Actidione HiVeg Agar Base with Actidione Composition per liter Glucose.50.0g Agar.15.0g Plant hydrolysate.5.0g Yeast extract.4.0g KH2PO4.0.55g KCl.0.425g CaCl22H2O.0.125g MgSO4-7H2O.0.125g Bromocresol Green.22.0mg MnSO4-4H2O.2.5mg FeCl3.2.5mg Cycloheximide solution.10.0mL pH 5.5 0.2 at 25 C Source This medium without actidione cycloheximide is available as a premixed powder from HiMedia. Cycloheximide Solution Composition per 10.0mL Cycloheximide.0.025g Preparation of Cycloheximide Solution Add cycloheximide to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Caution Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation. Preparation of Medium Add components except cycloheximide solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Aseptically add 10.0mL cycloheximide solution. Pour into sterile Petri dishes or leave in tubes. Use For the enumeration and detection of bacteria in specimens containing large numbers of yeasts and molds. Actidione HiVeg Agar Base without Actidione with .