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Handbook of Microbiological Media, Fourth Edition part 9

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Handbook of Microbiological Media, Fourth Edition part 9 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Alkaline Peptone Salt Broth 75 Alkaline Cellulose Agar Composition per liter Solution A.900.0mL Solution B.100.0mL Solution A Composition per 900.0mL Agar.15.0g Cellulose powder MN 300.15.0g NH4NO3.2.0g K2HPO4.1.0g Peptone.1.0g Yeast extract.0.5g CaCl2.0.4g MgSO4-7H2O.0.4g Preparation of Solution A Add components to distilled deionized water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 7.0 with 1jV HCl. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Solution B Composition per 100.0mL Na2CO3.0.5g Preparation of Solution B Add 0.5g of Na2CO3 to distilled deion-ized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 9.4 with 6 NaHCO3 solution. Autoclave for 15 min at 15 psi pres-sure-121 C. Cool to 50 -55 C. Preparation of Medium Aseptically combine 900.0mL of solution A with 100.0mL of solution B. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of cellulose-utilizing bacteria. Alkaline HiVeg Peptone Water Composition per liter NaCl.10.0g Plant peptone.10.0g pH 8.4 0.2 at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.5. Distribute into tubes or flasks. Autoclave for 10 min at 15 psi pressure-121 C. Use For the enrichment of Vibrio species from foods. Alkaline Nutrient Agar Composition per liter Agar.15.0g Peptone.5.0g Beef extract.3.0g pH 9.5-10.0 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically adjust to pH 9.5-10.0 with sterile 9 Na2CO3 solution. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of Bacillus alcalophilus and Bacillus species. Alkaline .