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Handbook of Microbiological Media, Fourth Edition part 15

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Handbook of Microbiological Media, Fourth Edition part 15 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Aolpha Medium 135 Yeast extract.10.0g Na2HPO4.1.0g pH 6.0 0.2 at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes. Use For for testing antimycotic sensitivity by the diffusion method. AO Agar Composition per liter Agar.11.0g Sodium acetate.0.5g Pancreatic digest of casein.0.5g Yeast extract.0.5 g Beef extract.0.2g pH 7.2 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the isolation and cultivation of Cytophaga species Herpeto-siphon species Saprospira species and Flexithrix species. AO Agar Composition per liter Agar.4.0g Sodium acetate.0.5g Pancreatic digest of casein.0.5g Yeast extract.0.5 g Beef extract.0.2g pH 7.2 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the maintenance of Cytophaga species Herpetosiphon species Saprospira species and Flexithrix species. AOAC Letheen Broth Association of Official Analytical Chemists Letheen Broth Composition per liter Peptic digest of animal tissue.10.0g Polysorbate 80.5.0g NaCl.5.0g Beef extract . 5.0g Lecithin.0.7g pH 7.0 0.2 at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into