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Handbook of Microbiological Media, Fourth Edition part 20

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Handbook of Microbiological Media, Fourth Edition part 20 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Bacillus selenitireducens Medium 185 Preparation of Medium Add components except sodium pyruvate solution to distilled deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.1. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Aseptically add sodium pyruvate solution. Aseptically distribute into sterile tubes or flasks. Use For the cultivation and maintenance of Bacillus schlegelii. Bacillus schlegelii Chemolithotrophic Growth Medium DSMZ Medium 261 Composition per liter Na2HPO4-2H2O.4.5g KH2PO4.1.5g NH4Cl.1.0g MgSO4-7H2O.0.2g MnSO4-2H2O.0.01g CaCl22H2O.0.01g Ferric ammonium citrate.5.0mg Trace elements solution SL-6.3.0mL pH 7.1 0.2 at 25 C Trace Elements Solution SL-6 Composition per liter MnCl24H2O.0.5g H3BO3.0.3g CoCl2-6H2O.0.2g ZnSO4-7H2O. 0.1g Na2MoO4-2H2O. 0.03g NiCl26H2O.0.02g CuCl22H2O.0.01g Preparation of Trace Elements Solution SL-6 Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.1. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the chemolithotrophic cultivation of Bacillus schlegelii. Incubation is at 65 C under an atmosphere of 5 O2 10 CO2 45 H2. Bacillus schlegelii Heterotrophic Growth Medium DSMZ Medium 260 Composition per liter Na2HPO4-2H2O.4.5g Na-pyruvate.1.5g KH2PO4.1.5g NH4Cl.1.0g MgSO4-7H2O.0.2g MnSO4-2H2O.0.01g CaCl22H2O.0.01g Ferric ammonium citrate.5.0mg Trace elements solution SL-6.3.0mL pH 7.1 0.2 at 25 C Trace Elements Solution SL-6 Composition per liter MnCl24H2O.0.5g H3BO3.0.3g CoCl2-6H2O.0.2g ZnSO4-7H2O. 0.1g Na2MoO4-2H2O. 0.03g NiCl26H2O.0.02g CuCl22H2O.0.01g Preparation of Trace Elements Solution SL-6 Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium Add components to distilled deionized water and bring volume to