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Handbook of Microbiological Media, Fourth Edition part 22

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Handbook of Microbiological Media, Fourth Edition part 22 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | B.D.G. Broth Hajna 205 L-Cysteine-HCTH2O Solution Composition per 10.0mL L-Cysteine-HCl-H2O.0.4g Preparation of L-Cysteine-HCTH2O Solution Add l-cysteine-HCl-H2O to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Fe4 P2O7 3-9H2O Solution Composition per 10.0mL Fe4 P2O7 3-9H2O.0.25g Preparation of Fe4 P2O7 3-9H2O Solution Add Fe4 P2O7 3-9H2O to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except Fe4 P2O7 3-9H2O solution L-cysteine-HCl-H2O solution and bovine serum albumin solution to distilled deionized water and bring volume to 970.0mL. Mix thoroughly. Adjust medium to pH 6.9 with 1N KOH. Heat gently and bring to boiling for 1 min. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Aseptically add 10.0mL of sterile bovine serum albumin solution the Fe4 P2O7 3-9H2O solution and the L-cysteine-HCl-H2O solution. Mix thoroughly. Pour into sterile Petri dishes with constant agitation to keep charcoal in suspension. Use For the isolation cultivation and maintenance of Legionella pneumophila and other Legionella species from environmental and clinical specimens. BCYEa without L-Cysteine Buffered Charcoal Yeast Extract Agar without L-Cysteine Composition per liter Agar.15.0g Yeast extract.10.0g ACES buffer 2- 2-amino-2-oxoethyl - amino -ethane sulfonic acid .10.0g Charcoal activated.2.0g a-Ketoglutarate.1.0g Fe4 P2O7 3-9H2O solution.10.0mL pH 6.9 0.2 at 25 C Fe4 P2O7 3-9H2O Solution Composition per 10.0mL Fe4 P2O7 3-9H2O.0.25g Preparation of Fe4 P2O7 3-9H2O Solution Add Fe4 P2O7 3-9H2O to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except Fe4 P2O7 3-9H2O solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Adjust medium to pH 6.9 with 1N KOH. Heat gently and bring to boiling for 1 min. Autoclave for 15 min at 15 psi pressure-121 C.