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Handbook of Microbiological Media, Fourth Edition part 27

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Handbook of Microbiological Media, Fourth Edition part 27 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Brewer Anaerobic Agar 255 Preparation of Selective Supplement Solution Add components to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except selective supplement solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Aseptically add selective supplement solution. . Mix thoroughly. Pour into Petri dishes or aseptically distribute into sterile tubes. Use For the cultivation of Brettanomyces spp. and the detection of this yeast in soft drinks. Brevibacillus levickii Medium DSMZ Medium 1064 Composition per liter Agar.18.0g CaCl2-2H2O.12.5g MnSO4-H2O.2.5g Yeast extract.2.0g KH2PO4.1.5g NH4 2SO4.1.25g MgSO4-7H2O.0.1g pH 5.5 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.5. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation ofBrevibacillus levickii. Brevibacterium Medium ATCC Medium 159 Composition per liter Agar.25.0g Glucose.20.0g CaCO3.20.0g Yeast extract.10.0g Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of Brevibacterium species. Brevibacterium Medium ATCC Medium 677 Composition per liter Agar.30.0g KH2PO4.2.0g Na2HPO4.2.0g NH4 2SO4.2.0g Yeast extract. 2.0g Tween 60.2.0g MgSO4-7H2O.0.2g FeSO4.7H2O.0.1g MnSO4.0.01g n-Hexadecane.50.0mL Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Blend for 30 min in a blender to disperse the n-hexadecane. Distribute into

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