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Handbook of Microbiological Media, Fourth Edition part 41

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Handbook of Microbiological Media, Fourth Edition part 41 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Clostridium alkalicellum Medium 395 Clostridium aldrichii Broth Composition per liter Cellobiose.10.0g NH4Cl.2.0g K2HPO4.1.65g NaCl.0.9g NH4 2SO4.0.9g L-Cysteine-HCl-H2O.0.5g KCl.0.5g MgSO4-7H2O.0.5g Trypticase .0.5g Yeast extract.0.5g Na2S-9H2O.0.2g CaCl2.0.09g Resazurin.0.5mg Wolfe s mineral solution.10.0mL Wolfe s vitamin solution.10.0mL NaHCO3.variable pH 7.0 0.2 at 25 C Wolfe s Mineral Solution Composition per liter MgSO4-7H2O.3.0g Nitrilotriacetic acid.1.5g NaCl.1.0g MnSO4-H2O.0.5g CaCl2.0.1g CoCl2-6H2O.0.1g FeSO4-7H2O.0.1g ZnSO4-7H2O.0.1g AlK SO4 212H2O.0.01g CuSO4-5H2O.0.01g H3BO3.0.01g Na2MoO4-2H2O.0.01g Preparation of Wolfe s Mineral Solution Add nitrilotriacetic acid to approximately 500.0mL of water and adjust to pH 6.5 with KOH to dissolve the compound. Bring volume to 1.0L with remaining water and add remaining compounds one at a time. Wolfe s Vitamin Solution Composition per liter Pyridoxine-HCl.10.0mg p-Aminobenzoic acid.5.0mg Lipoic acid.5.0mg Nicotinic acid.5.0mg Riboflavin.5.0mg Thiamine-HCl.5.0mg Calcium DL-pantothenate.5.0mg Biotin.2.0mg Folic acid.2.0mg Vitamin B12.0.1mg Preparation of Wolfe s Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium Prepare and dispense medium under 80 N2 20 CO2. Add components except NaHCO3 to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 3 min. Cool to room temperature while sparging with 80 N2 20 CO2. Add sufficient NaHCO3 to bring the pH to 7.0. Anaerobically distribute into tubes. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Clostridium aldrichii. Clostridium Alginate Medium Composition per liter of seawater Agar.15.0g Sodium alginate.10.0g K2HPO4.2.0g Peptone.1.0g Yeast extract.1.0g Seawater.1.0L pH 7.0-7.5 at 25 C Preparation of Medium Add K2HPO4 to 1.0L of seawater. Mix thoroughly. Gently heat while stirring to dissolve. Filter .