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Handbook of Microbiological Media, Fourth Edition part 43

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Handbook of Microbiological Media, Fourth Edition part 43 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Clostridium noterae Medium 415 Vitamin Solution Composition per liter Pyridoxine-HCl.10.0mg Calcium DL-pantothenate.5.0mg Lipoic acid.5.0mg Nicotinic acid.5.0mg p-Aminobenzoic acid.5.0mg Riboflavin.5.0mg Thiamine-HCl.5.0mg Biotin.2.0mg Folic acid.2.0mg Vitamin B12.0.1mg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium Add components except NaHCO3 solution and rhamnose solution to distilled deionized water and bring volume to 960.0mL. Mix thoroughly. Adjust pH to 6.5 with KOH. Sparge under 100 N2 for 3-4 min. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically add 20.0mL of sterile NaHCO3 solution and 20.0mL of sterile rhamnose solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile screw-capped bottles under 100 N2. Final pH of the medium should be 6.8. Use For the cultivation of Clostridium methylpentosum. Clostridium neopropionicum Medium Composition per liter KHCO3.4.0g Ethanol.1.0g NH4Cl.1.0g NaCl.0.6g Pancreatic digest of casein.0.5g Yeast extract.0.5 g KH2PO4.0.3g MgCl2-6H2O.0.1g CaCl2-2H2O.0.08g Resazurin.1.0mg Trace elements solution.10.0mL Vitamin solution.10.0mL L-Cysteine-HCl-H2O solution.10.0mL Na2S-9H2O solution.10.0mL pH 6.8 0.2 at 25 C Trace Elements Solution Composition per liter Nitrilotriacetic acid.12.8g FeCl3-6H2O.1.35g NaCl.1.0g NiCl26H2O.0.12g MnCl24H2O.0.1g CaCl22H2O.0.1g ZnCl2.0.1g Na2SeO3-5H2O.0.026g CuCl22H2O.0.025g CoCl26H2O.0.024g Na2MoO4-2H2O.0.024g H3BO3.0.01g Preparation of Trace Elements Solution Add nitrilotriacetic acid to approximately 500.0mL of distilled deionized water. Dissolve by adding KOH and adjust pH to 6.5. Add remaining components. Bring volume to 1.0L with additional distilled deionized water. Adjust pH to 7.0 with KOH. Vitamin Solution Composition per liter Pyridoxine-HCl.10.0mg Calcium DL-pantothenate.5.0mg Lipoic acid.5.0mg Nicotinic acid.5.0mg p-Aminobenzoic acid.5.0mg .