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Handbook of Microbiological Media, Fourth Edition part 57

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Handbook of Microbiological Media, Fourth Edition part 57 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Desulfovibrio giganteus Medium 555 swirl the medium to keep the grey precipitate suspended. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Desulfovibrio spp. Desulfovibrio gabonensis Medium Composition per 1002.0mL NaCl.50.0g MgCl2-6H20.3.3g Na2SO4.3.0g MgSO4-7H2O.1.6g KCl.0.3g NH4Cl.0.3g KH2PO4.0.2g CaCl22H2O.0.1g Yeast extract.0.1g Resazurin.0.5mg Sodium lactate solution.10.0mL NaHCO3 solution.10.0mL Na2S-9H2O solution.10.0mL Trace elements solution SL-10 with EDTA.1.0mL Seven vitamin solution.1.0mL pH 7.0-7.2 at 25 C Sodium Lactate Solution Composition per 10.0mL Sodium lactate.2.5g Preparation of Sodium Lactate Solution Add sodium lactate to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. NaHCO3 Solution Composition per 10.0mL NaHCO3.2.5g Preparation of NaHCO3 Solution Add NaHCO3 to distilled de-ionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Na2S-9H2O Solution Composition per 10.0mL Na2S-9H2O.0.2g Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Trace Elements Solution SL-10 with EDTA Composition per liter Disodium EDTA.3.0g FeCl24H2O.1.5g CoCl2-6H2O.190.0mg MnCl24H20.100.0mg ZnCl2.70.0mg Na2MoO4-2H2O.36.0mg NiCl26H2O.24.0mg H3BO3.6.0mg CuCl22H2O.2.0mg Preparation of Trace Elements Solution SL-10 with EDTA Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0. Seven Vitamin Solution Composition per liter Pyridoxine-HCl.0.3g Thiamine-HCl.0.2g Nicotinic acid.0.2g Calcium DL-pantothenate.0.1g Vitamin B12.0.1g p-Aminobenzoic acid.80.0mg Biotin.20.0mg Preparation of Seven Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter .