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Handbook of Microbiological Media, Fourth Edition part 61

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Handbook of Microbiological Media, Fourth Edition part 61 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Dichotomicrobium thermohalophilum Broth 595 Yeast extract.1.0g Artificial seawater 3x.960.0mL Hutner s basal salts solution.20.0mL NaHCO3 solution.20.0mL pH 7.0-7.2 at 25 C Artificial Seawater 3x Composition per liter NaCl.70.43g MgCl2-6H20.31.86g Na2SO4.11.75g CaCl22H2O.4.35g NaHCO3.2.88g KCl.1.99g KBr.0.29g H3BO3.0.08g Preparation of Artificial Seawater 3x Add components to dis-tilled deionized water and bring volume to 1.0L. Mix thoroughly. Hutner s Basal Salts Solution Composition per liter MgSO4-7H2O.29.7g Nitrilotriacetic acid.10.0g CaCl22H2O.3.335g FeSO4-7H2O.99.0mg NH4 6MoO7O24-4H2O.9.25mg Metals 44 .50.0mL Metals 44 Composition per 100.0mL ZnSO4-7H2O. 1.095g FeSO4-7H2O.0.5g Sodium EDTA.0.25g MnSO4-H2O.0.154g CuSO4-5H2O.39.2mg Co NO3 26H2O.24.8mg Na2B4O7-10H2O.17.7mg Preparation of Metals 44 Add sodium EDTA to distilled de-ionized water and bring volume to 90.0mL. Mix thoroughly. Add a few drops of concentrated H2SO4 to retard precipitation of heavy metal ions. Add remaining components. Mix thoroughly. Bring volume to 100.0mL with distilled deionized water. Preparation of Hutner s Basal Salts Solution Add nitrilotriacetic acid to 500.0mL of distilled deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled deionized water to 1.0L. Adjust pH to 6.8. NaHCO3 Solution Composition per 20.0mL NaHCO3.3.0g Preparation of NaHCO3 Solution Add NaHCO3 to distilled deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except NaHCO3 solution to distilled deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Aseptically add 20.0mL of sterile NaHCO3 solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and maintenance of Dichotomicrobium thermohalophilum. Dichotomicrobium thermohalophilum Broth Composition per liter .