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Handbook of Microbiological Media, Fourth Edition part 62

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Handbook of Microbiological Media, Fourth Edition part 62 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Double Sugar Agar Russell 605 Dorset Egg Medium Composition per liter Homogenized whole egg.950.0mL Glycerol.50.0mL pH 6.8-7.4 at 25 C Source This medium is available as a prepared medium from BD Diagnostic Systems. Homogenized Whole Egg Composition per liter Whole eggs.18-24 Preparation of Homogenized Whole Egg Use fresh eggs less than 1 week old. Scrub the shells with soap. Let stand in a soap solution for 30 min. Rinse in running water. Soak eggs in 70 ethanol for 15 min. Break the eggs into a sterile container. Homogenize by shaking. Filter through four layers of sterile cheesecloth into a sterile graduated cylinder. Measure out 1.0L. Preparation of Medium Filter sterilize glycerol. Combine glycerol and homogenized whole egg. Mix thoroughly. Distribute into sterile screw-capped tubes. Place tubes in a slanted position. Inspissate at 85 C moist heat for 45 min. Use For the maintenance of Mycobacterium species. Double-Strength Crude Lactobacillus Medium Composition per 1025.0mL Yeast extract Basamine .20.0g Sucrose.20.0g Casein hydrolysate.15.0g Potassium acetate.3.0g Histidine-HCl-H2O.2.0g Ascorbic acid.1.0g Pyridoxamine-HCl.33.0pg Salts solution A.20.0mL Salts solution B.5.0mL pH 5.4 0.2 at 25 C Salts Solution A Composition per liter K2HPO4-3H20.16.5g KH2PO4-H2O.16.5g Preparation of Salts Solution A Add components to distilled de-ionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Salts Solution B Composition per liter MgSO4-7H2O.8.0g FeSO4-7H2O.0.4g NaCl.0.4g HCl concentrated.0.1mL MnSO4-H2O.0.1mL Preparation of Salts Solution B Add components to distilled de-ionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Add components except salts solution A and salts solution B to distilled deionized water and bring volume to 975.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add 20.0mL of sterile salts solution A