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Handbook of Microbiological Media, Fourth Edition part 69

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Handbook of Microbiological Media, Fourth Edition part 69 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Ferrous Sulfate Yeast Extract Medium 675 Vitamin Solution Composition per liter Pyridoxine-HCl.10.0mg Thiamine-HCl-2H2O.5.0mg Riboflavin.5.0mg Nicotinic acid.5.0mg D-Ca-pantothenate.5.0mg p-Aminobenzoic acid.5.0mg Lipoic acid.5.0mg Biotin.2.0mg Folic acid.2.0mg Vitamin B12.0.1mg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80 H2 20 CO2. Filter sterilize. Na2S-9H2O Solution Composition per 10.0mL Na2S-9H2O.0.5g Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled deionized water and bring volume to 10.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Store anaerobically. Preparation of Medium Add components except Na2S-9H2O solution vitamin solution and Na-pyruvate solution to distilled deion-ized water and bring volume to 990.0mL. Mix thoroughly. Flush medium with N2 for 20 min. Adjust medium pH to 7.0 with 4.V H2SO4. Add 10.0mL of Na2S-9H2O solution. Mix thoroughly. Readjust the medium pH to 7.0 with H2SO4 while flushing the gas phase only with N2. Dispense 10mL volumes into 100mL serum bottles with rubber stoppers under N2. Replace gas phase by 80 H2 20 CO2 gas mixture and finally pressurize the bottles to 2 bar gas overpressure. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically inject via syringe 0.1mL vitamin solution and 0.1mL Na-pyruvate solution into each tube containing 10.0mL of the autoclaved medium. Use For the cultivation of Ferroglobus placidus. Ferroplasma acidiphilum Medium DSMZ Medium 874 Composition per 1001.6mL Solution A.950.0mL Solution B.10.0mL Solution C.1.6mL pH 1.7 0.2 at 25 C Solution A Composition per 950.0mL MgSO4-7H2O.0.4g NH4 2SO4.0.2g KCl.0.1g K2HPO4.0.1g Preparation of Solution A Add components to distilled deionized water and bring volume to 950.0mL. Mix thoroughly. Solution B Composition per 50.0mL FeSO4-7H2O.25.0g H2SO4 1V.40.0mL Preparation of Solution B Add components to distilled deionized water