Đang chuẩn bị liên kết để tải về tài liệu:
Handbook of Microbiological Media, Fourth Edition part 70

Không đóng trình duyệt đến khi xuất hiện nút TẢI XUỐNG Tải xuống

Handbook of Microbiological Media, Fourth Edition part 70 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Flexibacter Broth 685 Sodium tartrate.1.77g FeSO4-7H2O.1.36g CoCl2-6H2O.0.04g CuCl2.2H2O.0.027g Na2MoO4-2H2O.0.025g ZnCl2.0.02g Preparation of Trace Elements Solution HO-LE Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except traqce elements solution HO-LE to filtered seawater and bring volume to 999.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add 1.0mL of sterile trace elements solution HO-LE. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and maintenance of Flexibacter polymorphus. Flexibacter Agar LMG Medium 60 Composition per liter Agar.15.0g Casamino acids.1.0g Tris buffer.1.0g MgSO4-7H2O.0.1g KNO3.0.1g CaCl22H2O.0.1g Sodium-P-glycerophosphate.0.1g Thiamine.1.0mg Vitamin B12.1.0pg Glucose solution.10.0mL Vitamin solution.10.0mL Trace elements solution.1.0mL pH 7.5 0.2 at 25 C Glucose Solution Composition per 100.0mL Glucose.10.0g Preparation of Glucose Solution Add glucose to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Trace Elements Solution Composition per liter H3BO3.2.85g FeSO4-7H2O.2.49g MnCl24H2O.1.8g Sodium tartrate.1.77g CaCl22H2O.40.4mg CuCl22H2O.26.9mg ZnCl2.20.8mg Na2MoO4-2H2O.25.2mg Preparation of Trace Elements Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Vitamin Solution Composition per 10.0mL Thiamine.10.0mg Vitamin B12.10.0pg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except vitamin solution glucose solution and trace elements solution to 979.0mL distilled deionized water. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C.