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Handbook of Microbiological Media, Fourth Edition part 78

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Handbook of Microbiological Media, Fourth Edition part 78 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | GV Medium 765 Trace Elements Solution SL-10 Composition per liter FeCl2-4H2O.1.5g CoCl26H2O.190.0mg MnCl24H20.100.0mg ZnCl2.70.0mg Na2MoO4-2H2O.36.0mg NiCl26H2O.24.0mg H3BO3.6.0mg CuCl2-2H2O.2.0mg HCl 25 solution .10.0mL Preparation of Trace Elements Solution SL-10 Add FeCl2-4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Gas under 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Prepare and dispense medium under 80 N2 20 CO2. Add components except substrate solution MgSO4-7H2O solution CaCl2-2H2O solution selenite-tungstate solution L-cysteine-HCl solution and Na2S-9H2O solution to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically add 10.0mL of sterile substrate solution 10.0mL of sterile MgSO4-7H2O solution 10.0mL of sterile CaCl2-2H2O solution 1.0mL of sterile selenite-tungstate solution 10.0mL of sterile L-cysteine-HCl solution and 10.0mL of sterile Na2S-9H2O solution. Mix thoroughly. Adjust pH to 7.0-7.2. Aseptically and anaerobically distribute into sterile tubes or flasks. Use For the cultivation and maintenance of Clostridium magnum and Clostridium species. GV Medium Composition per 1061.0mL NaHCO3.3.0g NaCl.2.25g Yeast extract.1.0g NH4Cl.0.5g K2HPO4.0.348g KH2PO4.0.227g FeSO4-7H2O.2.0mg Resazurin.0.5mg Substrate solution.20.0mL Vitamin solution.10.0mL L-Cysteine-HCl solution.10.0mL Na2S-9H2O solution.10.0mL MgSO4-7H2O solution.10.0mL CaCl2-2H2O solution.10.0mL Selenite-tungstate solution.1.0mL Trace elements solution SL-10.1.0mL pH 7.0 0.2 at 25 C Substrate Solution Composition per 20.0mL Sodium citrate.6.0g Preparation of Substrate Solution Add sodium citrate to distilled deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pres-sure-121 C. .