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Handbook of Microbiological Media, Fourth Edition part 79

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Handbook of Microbiological Media, Fourth Edition part 79 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Haliscomenobacter hydrossis Medium 775 Hagem s Modess Medium Composition per liter Agar noble.15.0g Glucose.10.0g DL-Asparagine.1.0g Yeast extract.1.0g Peptone.1.0g MgSO4-7H2O.0.5g KH2PO4.0.35g K2HPO4.0.15g Thiamine-HCl.40.0mg FeCl3-6H2O or ferric citrate.1.0mg Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of Agaricus macrosporus Boletus rubinellus Flammulina velutipes Inonotus hispidus Lactarius turpis Nodulisporium tuberum Odontia bicolor Phellinus pomaceus Phellinus tremulus Phellinus weirii Phlebia gigantea Poria medula-panis Pterid-iospora spinosispora Schizophyllum commune Thanatephorus cucum-eris Thelephora terrestris Trametes versicolor Tuber albidum and Tuber rufum. Half Fraser Broth Composition per liter NaCl.20.0g Na2HPO4.12.0g Proteose peptone . 5.0g Tryptone.5.0g Lab Lemco powder.5.0g LiCl.3.0g KH2PO4.1.35g Esculin.1.0g Half Fraser supplement solution.10.0mL pH 7.2 0.2 at 25 C Source This medium is available as a premixed powder from Oxoid Unipath. Half Fraser Supplement Solution Composition per 10.0mL Ferric ammonium citrate.0.5 g Acriflavme-HCl.0.125g Nalidixic acid.0.05g Ethanol.5.0mL Preparation of Half Fraser Supplement Solution Add components to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except h alf Fraser supplement solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile half Fraser supplement solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the isolation of Listeria species from food and environmental species. A .