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Handbook of Microbiological Media, Fourth Edition part 89

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Handbook of Microbiological Media, Fourth Edition part 89 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Inorganic Salts-Maltose Medium 875 Glucose.1.0g KCl.0.4g CaCl2-2H2O.0.265g MgSO4-7H2O.0.2g NaH2PO4-H2O.0.14g Preparation of Earle s Balanced Salts Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium Combine components. Mix thoroughly. Filter sterilize. Store at 4 -10 C. Use For the screening of Escherichia coli for pathogenicity using the HeLa cell test for invasiveness. Infusion Agar See Blood Agar Base Infusion Broth Composition per liter Pancreatic digest of casein.13.0g NaCl.5.0g Yeast extract.5.0g Heart muscle solids from infusion.2.0g pH 7.4 0.2 at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of a wide variety of microorganisms. Infusion Cystine Agar Base HiVeg Composition per liter Agar.15.0g Glucose.10.0g Plant infusion.10.0g Plant peptone No. 3.10.0g NaCl.5.0g L-Cystine.1.0g pH 7.2 0.2 at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Add components to distilled deionized water and bring volume to1.0L. Mix thoroughly. Gently heat and bring to boiling with frequent agitation. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes. Use For the cultivation of Gram-negative cocci and other pathogenic organisms. Infusion Cystine Agar Base HiVeg with Hemoglobin Composition per liter Agar.15.0g Glucose.10.0g Plant infusion.10.0g Plant peptone No. 3.10.0g NaCl.5.0g L-Cystine.1.0g Hemoglobin solution 2 .100.0mL pH 7.2 0.2 at 25 C Source This medium without hemoglobin solution is available as a premixed powder from HiMedia. Preparation of Medium Add components except hemoglobin solution to distilled deionized water and bring volume to