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Handbook of Microbiological Media, Fourth Edition part 92

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Handbook of Microbiological Media, Fourth Edition part 92 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Kohn Two Tube HiVegMedium No. 1 Base with Urea 905 sterile Petri dishes. Allow plates to dry in the dark at 30 C for 24 hr before using. Use For the isolation and cultivation of acidogenic microorganisms from foods. For the differentiation of citrate-fermenting lactic bacteria such as Lactobacillus lactis subspecies diacetylactis from the nonci-trate-fermenting Lactobacillus lactis subspecies cremoris. Knisely Medium for Bacillus anthracis Composition per liter Beef heart solids from infusion.500.0g Agar.15.0g Pancreatic digest of casein.10.0g NaCl.5.0g EDTA.200.0mg Lysozyme.40.0mg Thallous acetate.40.0mg Polymyxin.30 000U Preparation of Medium Add components except EDTA lysozyme thallous acetate and polymyxin to distilled deionized water and bring volume to 1.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.3. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile EDTA lysozyme thallous acetate and polymyxin. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and maintenance of Bacillus anthracis. Koch s K1 Medium Composition per liter Glucose.1.8g Peptone.0.6g Yeast extract.0.4g Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of a variety of fungi. Kohn Two Tube Medium No. 1 Base with Urea Composition per liter Agar.16.0g Peptic digest of animal tissue.15.0g Mannitol.10.0g Beef extract.2.0g Yeast extract.2.0g Glucose.1.0g Phenol Red.0.05g Urea solution.25.0mL pH 7.3 0.2 at 25 C Source This medium without urea solution is available as a premixed powder from HiMedia. Urea Solution Composition per 100.0mL Urea.5.0g Preparation of Urea Solution Add urea to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components to distilled .