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Handbook of Microbiological Media, Fourth Edition part 103

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Handbook of Microbiological Media, Fourth Edition part 103 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Marine Flagellate Medium with B-Vitamins 1015 sterile CaCl2-2I I2O solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation of Cytophaga species Flexibacter species Microscilla species and Saprospira grandis. Marine Cytophaga Medium A Composition per liter Agar.15.0g Pancreatic digest of casein.2.0g Beef extract.0.5g Yeast extract.0.5g Sodium acetate.0.2g Seawater.700.0mL pH 7.2 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation of Flexibacter maritimus. Marine Cytophaga Medium B Composition per liter Agar.15.0g Pancreatic digest of casein.2.0g Beef extract.0.5g Yeast extract.0.5g Sodium acetate.0.2g Seawater.500.0mL pH 7.2 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation of Vibrio ordalii. Marine Cytophaga Medium C Composition per liter Agar.15.0g Pancreatic digest of casein.2.0g Beef extract.0.5g Yeast extract.0.5g Sodium acetate.0.2g pH 7.2 0.2 at 25 C Preparation of Medium Add components to seawater and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation of Cytophaga agarovorans Cytophaga fer-mentans and Cytophaga salmonicolor. Marine Desulfovibrio Medium Composition per liter Solution A.980.0mL Solution B.10.0mL Solution C.10.0mL pH 7.8 0.2 at 25 C Solution A Composition per 980.0mL NaCl.25.0g DL-Sodium lactate.2.0g .