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Handbook of Microbiological Media, Fourth Edition part 117

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Handbook of Microbiological Media, Fourth Edition part 117 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Micro Assay Culture Agar 1155 CaCl2.0.36g NaBr.0.026g NaHCO3 solution.10.0mL pH 7.5 0.2 at 25 C NaHCO3 Solution Composition per 10.0mL NaHCO3.0.06g Preparation of NaHCO3 Solution Add NaHCO3 to distilled de-ionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except NaHCO3 solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add 10.0mL of sterile NaHCO3 solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation of Zygomonas mobilis Salinicoccus hispani-cus Salinicoccus roseus and Pseudomonas beijerinckii. MH Medium 15 LMG Medium 258 Composition per liter NaCl.121.5g MgSO4-7H2O.14.4g MgCl2.10.5g Yeast extract.10.0g Proteose peptone No.3.5.0g KCl.3.0g Glucose.1.0g CaCl2.0.54g NaBr.39.0mg NaHCO3 solution.10.0mL pH 7.5 0.2 at 25 C NaHCO3 Solution Composition per 10.0mL NaHCO3.0.09g Preparation of NaHCO3 Solution Add NaHCO3 to 10.0mL of distilled deionized water. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except NaHCO3 solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add 10.0mL sterile NaHCO3 solution. Aseptically distribute into sterile tubes or flasks. Use For cultivation and maintenance of Bacillus halophilus. MH Salts Composition per liter NaCl.120.5g MgCl26H2O.22.4g Agar . 20.0g MgSO4.14.4g Yeast extract.10.0g Proteose peptone No. 3.5.0g KCl.3.0g Glucose.1.0g CaCl2.0.54g NaHCO3.0.09g NaBr.0.039g pH 7.5 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of Bacillus .