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Handbook of Microbiological Media, Fourth Edition part 119

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Handbook of Microbiological Media, Fourth Edition part 119 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Mineral Medium with Dichloromethane 1175 Nicotinamide.25.0mg Biotin.20.0mg Folic acid.20.0mg Pyridoxamine-HCl.10.0mg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Cyanuric Acid Solution Composition per 10.0mL Cyanuric acid.645.0mg Preparation of Cyanuric Acid Solution Add cyanuric acid to 10.0mL distilled deionized water. Mix thoroughly. Adjust pH to 7.0. Filter sterilize. Glycerol Solution Composition per 10.0mL Glycerol.5.5g Preparation of Glycerol Solution Add glycerol to distilled de-ionized water. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except cyanuric acid solution glycerol solution and vitamin solution to 1.0L distilled deionized water. Adjust pH to 7.25. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add 10.0mL cyanuric acid solution 10.0mL glycerol solution and 10.0mL vitamin solution. Mix thoroughly. Aseptically distribute to sterile tubes or flasks. Use For the cultivation of Gordonia rubripertincta Rhodococcus rubropertinctus and other cyanuric acid-utilizing bacteria. Mineral Medium with Dichlorobenzoate Composition per liter Na2HPO4.2.78g KH2PO4.2.78g NH4 2SO4.1.0g Hutner s mineral base.20.0mL 2 4-Dichlorobenzoate solution.10.0mL pH 6.8 0.2 at 25 C Hutner s Mineral Base Composition per liter MgSO4-7H2O.29.7g Nitrilotriacetic acid.10.0g CaCl22H2O.3.34g FeSO4-7H2O.99.0mg NH4 2MoO4.9.25mg Metals 44 .50.0mL Preparation of Hutner s Mineral Base Add nitrilotriacetic acid to 500.0mL of distilled deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Readjust pH to 7.2 with H2SO4 or KOH. Add distilled deionized water to 1.0L. Metals 44 Composition per 100.0mL ZnSO4-7H2O.1.1g FeSO4-7H2O.0.5g EDTA.0.25g MnSO4-7H2O.0.154g CuSO4-5H2O.0.04g Co NO3 26H2O.0.025g Na2B4O7-10H2O.0.018g Preparation of Metals 44 Add a few drops of H2SO4 to dis-tilled deionized water to inhibit precipitate formation. Add .