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Handbook of Microbiological Media, Fourth Edition part 120

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Handbook of Microbiological Media, Fourth Edition part 120 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Mineral Salts Peptonized Milk Agar 1185 Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Rhodococcus rhodo-chrous. Mineral Salts Medium with Methanol Composition per liter NaNH4HPO4-4H2O.1.74g NaH2PO4-H2O.0.54g MgSO4-7H2O.0.2g KCl.0.04g FeSO4-7H2O.5.0mg Methanol .5.0mL Trace minerals solution.1.0mL pH 7.2 0.2 at 25 C Trace Minerals Solution Composition per liter H3BO3.2.86g MnCl24H20.1.81g ZnSO4-7H2O.0.22g CuSO4-5H2O.0.08g CoCl2-6H2O.0.06g Na2MoO4-2H2O.25.0mg Preparation of Trace Minerals Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium Add components except methanol to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Filter sterilize methanol. Aseptically add sterile methanol to cooled sterile basal medium. Use For the cultivation and maintenance of Rhodococcus rhodo-chrous. Mineral Salts Medium with Methanol and Yeast Extract Composition per liter NaNH4HPO4-4H2O.1.74g NaH2PO4-H2O.0.54g MgSO4-7H2O.0.2g Yeast extract.0.2g KCl.0.04g FeSO4-7H2O.5.0mg Methanol.5.0mL Trace minerals solution.1.0mL pH 7.2 0.2 at 25 C Trace Minerals Solution Composition per liter H3BO3.2.86g MnCl24H20.1.81g ZnSO4-7H2O.0.22g CuSO4-5H2O.0.08g CoCl2-6H2O.0.06g Na2MoO4-2H2O.25.0mg Preparation of Trace Minerals Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium Add components except methanol to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Filter sterilize methanol. Aseptically add sterile methanol to cooled sterile basal medium. Aseptically distribute into sterile tubes or flasks. Use For .